Basic Segmentation and Quantification#
In this section, we are going to make a macro to do some basic segmentation and intensity quantification on an image. We will do this on a single image, but you can imagine using what we will learn to process many images!
Learning Objectives
- Learn how to deal with multi-channel images and multiple images
- Learn how to apply a threshold to an image
- Learn how to use Regions Of Interest (ROIs) in a script and perform quantifications
- Learn how to use the ROI manager